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KMID : 1188320120060030381
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Gut and Liver
2012 Volume.6 No. 3 p.381 ~ p.387
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Involvement of Nuclear Factor Kappa B in High-Fat Diet-Related Pancreatic Fibrosis in Rats
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Ming-Xian Yan
Ren Hong-Bo
Yi Kou
Min Meng
Yan-Qing Li
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Abstract
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Background/Aims:High-fat diets contribute to pancreatic fibrogenesis, but the pathogenesis remains unclear. This study investigated the role of nuclear factor kappa B (NF-κB) in high-fat diet-induced pancreatic fibrosis in rats.
Methods:Male Wistar rats were fed a high-fat diet or standard normal chow for 20 weeks. Pancreatic fibrosis was determined by Sirius red staining. Immunohistochemical staining, reverse transcription-polymerase chain reaction and Western blotting were used to identify NF-κB-associated genes or protein expressions.
Results:Inflammation, fat deposition, pancreatic stellate cell activation and fibrosis were observed in the pancreases of the high-fat diet group. NF-κB subunit p65 (NF-κB/p65) expression was localized to the nucleus, and intercellular adhesion molecule 1 (ICAM-1) was over-expressed. Pancreatic gene expression levels of NF-κB/p65, ICAM-1 and tumor necrosis factor α were all elevated significantly in rats fed a high-fat diet compared with control rats. Western blotting also revealed significantly increased levels of ICAM-1 and nuclear NF-κB/p65 in rats fed high-fat diets comparison with control rats.
Conclusions:NF-κB is involved in high-fat diet-related pancreatic fibrosis.
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KEYWORD
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High-fat diet, Pancreatic fibrosis, NF-kappa B, Intercellular adhesion molecule 1, Tumor necrosis factor-alpha
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